In chromatography, there is a mobile phase and a stationary phase. The stationary phase is usually a solid, and the mobile phase is usually a liquid or even a gas. As the mobile phase moves over the stationary phase, it takes some of the components in the mixture along with it, but at different rates. The rate depends on how strongly the mixture components stick to the stationary phase. Here, we use this technique to separate different components of the blood, for therapeutic use.
In this technique, we use two phases as mobile phase and stationary phase. Accordingly, the mobile phase should contain our sample, and the stationary phase helps to separate it into components. We pass the mobile phase with the sample through the stationary phase where the components in the sample move at different speeds. This causes the components to separate.
Therefore, the theory behind the technique is the differential partitioning of components between the mobile and stationary phases. Electrophoresis is a laboratory technique that we use to analyze a sample using the electrical properties of the chemical species present in that sample whereas chromatography is an analytical technique that we use to analyze samples using the partition coefficients of chemical species present in the sample.
Thus, this is the major difference between electrophoresis and chromatography. Moreover, a difference between electrophoresis and chromatography based on usage is that we can use chromatography for liquid, solid and gaseous compounds whereas we generally carry out electrophoresis on liquid and solid compounds only. Chromatography paper is used to separate chemicals while filter paper is used to separate liquid and solid.
Paper chromatography separate liquids on special paper. Paper chromatography is a classical example of partition chromatography as the separation of the analyte occurs by the process of partition between the water molecules present in the interstices of the cellulose of which the paper is made of serving as liquid stationary phase and any solvent used as mobile phase. Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
There are two types of paper chromatography: 1. Ascending Chromatography 2. Descending Chromatography. There is no such thing as grass chromatography. If you like science Paper Chromatography is something to look into. You can take Chemistry classes to find out different ideas on how to use Paper Chromatography.
Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works. A mixture of various inks can be separated out by paper chromatography. Whatman Paper. Paper Chromatography is a slow technique as compared to others.
It is not sensitive as Thin Layer Chromatography. The components tend to streak out as the solvent reaches the front. Paper chromatography is used as an analytical method not for production. There are two categories of electrophoresis which are used to test a sample. These two types are decided to depend on the type of ion that is present in the sample to be tested.
These two types are known as cataphoresis and anaphoresis. The cataphoresis is used mainly for the positively charged ions present in the sample, which is to be tested, and on the other hand, the anaphoresis is used mainly for the negatively charged ions present in the sample, which is to be tested. When it comes to the processing of human blood, the chromatography technique plays a very important role in it.
In this, the chromosome technique is used to separate the different components present in the human blood for therapeutic use. There are two faces in this technique which are the mobile phase and stationary phase.
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